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KMID : 0379119870150040269
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Korean Journal of Mycology
1987 Volume.15 No. 4 p.269 ~ p.270
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Toxic metabolites produced by Helminthosporium sativum (Cochliobolus sativus)
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Lee Sang-Sun
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Abstract
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A modified toxin production broth was compared with a previously employed broth for the production of toxic metabolites from Helminthosporium sativum. Asparagine and organic acids of the previous broth are known to inhibit the growth of lettuce and wheat seedlings, and this has complicated the study of toxin production. These components were completely separated from the broth by passage through a C-18 column. Use of an octadecyl silane affinity column allowed separation of a relatively pure preparation of the toxic metabolites from the culture broth with 97% recovery.
Solvent extraction of the toxic metabolites indicated two kinds of toxins. Characterization studies of the toxin suggested that the diethyl ether insoluble toxin (M) was a small peptide that caused necrosis and stunting in lettuce, wheat and barley seedlings. The diethyl ether soluble toxin (D) was related structurally to helminthosporal, and caused stunting without necrosis. Wheat and barley lines showed differential sensitivity to this component. In some respects toxin production by H. sativum was similar to that from the related pathogens, H. carbonum and H. victoriae. The proportion of the two toxins produced by different H. sativum isolates differed.
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